CHEMICALS FOR AFFINITY CHROMATOGRAPHY

Brand	Owner (click to sort)	Address	Description
AFFI-GEL	Bio-Rad Laboratories, Inc.	1000 Alfred Nobel Drive Hercules CA 94547	CHEMICALS FOR AFFINITY CHROMATOGRAPHY, ENZYME IMMOBILIZATION AND IMMUNOSORPTION;
STEROGEL	KOVER, GABOR	150 HOME ST. SOMERSET NJ 08873	CHEMICALS FOR AFFINITY CHROMATOGRAPHY;
	Where the owner name is not linked, that owner no longer owns the brand

Technical Examples

A process is provided for the preparation of albumin which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, host proteins, fragments of albumin, polymers or aggregates of albumin and viruses, and which is essentially non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises passing albumin (preferably expressed and secreted by transformed yeast) through positive mode cation exchange and then positive mode anion exchange chromatography. Other steps may also be employed, ultrafiltration, gel permeation chromatography, affinity chromatography binding the albumin by using blue dyes) and affinity chromatography binding contaminants by using an aminophenylboronic acid resin. Elution of albumin, with a compound having affinity for albumin, from a material having no specific affinity for albumin is also disclosed, as is removal of ammonium ions with a counter-ion.