APPARATUS FOR ELECTROPHORESIS ACCESSORIES

Brand	Owner	Address	Description
DESAPHOR	C. Desaga GmbH, Nachf. Erich Fecht	Maaszstrasse 26-28 69 Heidelberg Germany	Apparatus for Electrophoresis and Accessories Therefor-Namely, Measuring Chambers, Electrodes, Gels, Carrier Plates, Cooling Plates, Buffer Bridges, Bridge Holders, Diffusion Barriers, Membranes, Membrane Holders, Sample Holders, Standard Plates, Microtiter Plates: Preparation Frames, Punching Blades, Safety Covers, Well Formers Syrings, Gel Troughs, Application Paper and Fleece, Cuvettes, Application Stripes, Electrode Stripes, Printing Paper, Rubber Roller, Separation Foils, Spray Guns;
	Where the owner name is not linked, that owner no longer owns the brand

Technical Examples

Methods of use, accessories and chambers, optimal for performing Pulsed Field Gel Electrophoresis (PFGE) of DNA molecules in 'Contour Clamped Homogeneous Electric Field' (CHEF) and 'Transversal Alternating Field Electrophoresis' (TAFE) systems, are provided herein. DNA molecules are rapidly separated in the minigels of these chambers. The sizes of chambers and accessories are determined by the separation between the opposite polarity electrodes; which is comprised between 6.2 and 15 cm. Reproducibility of molecule separation is achieved because the accessories warrant homogeneous electric resistance in the buffer and minigels. Chambers allow a high-throughput sample format using the reagents efficiently. It is attained excluding the non-useful electrophoresis zones For a better optimization, TAFE chambers have several useful electrophoresis zones (UEZ), each carrying a minigel. One or more UEZ can be activated at will in the electrophoresis, to vary the number of minigels, the number of samples and the amount of buffer among the experiments. TAFE chambers having 'inverted electrode configuration' with the cathodes at their bottom are presented.